A Rapid and Facile Separation-Detection Integrated Strategy for Exosome Profiling Based on Boronic Acid-Directed Coupling Immunoaffinity.

Exosomes are a promising noninvasive tumor biomarker for cancer diagnosis and classification. However, efficient capture and precise analysis of exosomes in complex biological samples remain challenging. Here, sensitive profiling of exosomes with an integrated separation-detection strategy of 37 min is performed based on boronic acid-directed coupling immunoaffinity. The modification of g-C3N4 nanosheets with boronic acid (BCNNS) contributes to antibody binding under physiological conditions, which is accompanied by fluorescence enhancement. When exosomes are captured by an antibody equipped with BCNNS, a decrease in fluorescence can be induced; moreover, using the dispersion property of BCNNS, the exosomes can be separated by a simple centrifugation step. The protocol shows a favorable sensitivity with a detection limit of 2484 particles/mL. By changing only the fused antibody, exosome phenotype information profiling can be achieved, and exosomes derived from four different cell lines (HeLa, HepG2, MCF-7, and MCF-10A) can be successfully distinguished. More significantly, the positive prediction accuracy results reach 100% for serum samples from different individuals and have the advantage of multiple parameters; thus, the method has great potential in noninvasive diagnosis and point-of-care testing.

[Determination of Na, Mn, Mg, Cr and Ni in 10 Chinese traditional medicines by FAAS].

Five metallic elements in 10 Chinese traditional medicines such as Na, Mn, Mg, Cr and Ni were determined by flame atomic absorption spectrophotometer. The results show that there are rich metal elements, but the contents are different in 10 Chinese traditional medicines. Among them, the content of Na element is the highest in Naosaitong; the content of milligram is the highest in Zhongfenghuichunpian; the content of Mn is the highest in Qianglitianmaduzhong pill; but in Xiaoshuantongluo capsule, the contents of Cr and Ni are higher. And it provide useful data for discussing between trace elements in Chinese traditional medicines and the cure for cerebrovascular.

[Analysis of K, Na, Ca and Mg in yingpingwanbixiao and yingpingwanbitong].

The contents of K, Na, Ca and Mg in three Yingpingwanbixiao and one Yingpingwanbitong were determined by FAAS. The results showed that the contents of K, Na, Ca and Mg in Yingpingwanbixiao and Yingpingwanbitong were all abundant. This paper provided useful data for the relationship between the therapeutic efficacy and the contents of magnificence elements in Yingpingwanbixiao and Yingpingwanbitong.